环境化学与生态毒理学国家重点实验室

近期成果

1.     A Simple Aptamer Molecular Beacon Assay for Rapid Detection of Aflatoxin B1, Chao Wang, LinLin Sun, Qiang Zhao*, Chinese Chemical Letter, 2019, 30, 1017-1020.

2.     Affinity Interactions by Capillary Electrophoresis: Binding, Separation, and Detection, Fangzhi Yu, Qiang Zhao, Dapeng Zhang, Zheng Yuan, Hailin Wang*, Analytical Chemistry, 2019, 91, 372–387

3.     Aptamer-Structure Switch Coupled with Horseradish Peroxidase Labeling on a Microplate for the Sensitive Detection of Small Molecules, Yapiao Li, Linlin Sun, Qiang Zhao*, Analytical Chemistry, 2019, 91, 2615-2619

4.     Nucleic Acid Aptamers Improving Fluorescence Anisotropy and Fluorescence Polarization Assays for Small Molecules, Qiang Zhao*, Jeffrey Tao, Jagdeesh S. Uppal, Hanyong Peng, Hailin Wang, X. Chris Le*, Trends in Analytical Chemistry, 2019, 110, 401-409.

5.     A Simple Fluorescent Aptamer Based Assay Coupled with Fluorescence Scanning Capillary Array for Aflatoxin B1, Linlin Sun, Qiang Zhao*, Analyst, 2018, 143, 4600-4605.

6.     Direct Fluorescence Anisotropy Approach for Aflatoxin B1 Detection and Affinity Binding Study by Using Single Tetramethylrhodamine Labeled Aptamer, Linlin Sun, Qiang Zhao*, Talanta, 2018, 189, 442-450.

7.     Development of Aptamer Fluorescent Switch Assay for Aflatoxin B1 by Using Fluorescein-Labeled Aptamer and Black Hole Quencher 1-Labeled Complementary DNA, Yapiao Li, Linlin Sun, Qiang Zhao*, Analytical Bioanalytical Chemistry 2018, 410, 6269-6277.

8.     Crystal Structures of Thrombin in Complex with Chemically Modified Thrombin DNA Aptamers Reveal the Origins of Enhanced Affinity, Rafal Dolot, Curtis H. Lam, Malgorzata Sierant, Qiang Zhao, Feng-Wu Liu, Barbara Nawrot, Martin Egli,* Xianbin Yang* Nucleic Acids Research 2018, 46, 4819-4830.

9.     Competitive Horseradish Peroxidase-Linked Aptamer Assay for Sensitive Detection of Aflatoxin B1, Linlin Sun, Qiang Zhao*, Talanta, 2018, 179, 344-349.

10.  Detection of Human Neutrophil Elastase by Aptamer Affinity Capillary Electrophoresis Coupled with Laser-induced Fluorescence Using Specified Site Fluorescently Labeled Aptamer, Yunlong Bai, Hailin Wang, Qiang Zhao*, Analytical Bioanalytical Chemistry , 2017, 409, 6843-6849.

11.  Investigation of Effects of Metal Ions in Sample Buffer on Capillary Electrophoresis Coupled with Laser-Induced Fluorescence Analysis of Thrombin Using Dye-Labeled 29-mer DNA Aptamer, Yunlong Bai, Qiang Zhao*, Analytical Methods, 2017, 9, 5684-5690.

12.  Enhancing the Affinity of Anti-Human α-Thrombin 15-mer DNA Aptamer and Anti-Immunoglobulin E Aptamer by PolyT Extension, Yunlong Bai, Yapiao Li, Dapeng Zhang, Hailin Wang, Qiang Zhao*, Analytical Chemistry, 2017, 89, 9467-9473.

13.  Rapid Fluorescence Detection of Immunoglobulin E Using an Aptamer Switch Based on Binding-Induced Pyrene Excimer, Yunlong Bai, Qiang Zhao*, Analytical Methods, 2017, 9, 3962-3967.

14.  Aptamer based surface plasmon resonance sensor for aflatoxin B1, Linlin Sun, Liqing Wu, Qiang Zhao*, Microchimica Acta, 2017, 184, 2605-2610.

15.  Competitive fluorescence anisotropy/polarization assay for ATP using aptamer as affinity ligand and dye-labeled ATP as fluorescence tracer, Yapiao Li, Linlin Sun, Qiang Zhao*, Talanta, 2017, 174, 7-13.

16.  Direct fluorescence anisotropy assay for cocaine using tetramethylrhodamine-labeled aptamer, Yingxiong Liu, Qiang Zhao*, Analytical Bioanalytical Chemistry, 2017, 409, 3993-4000.

17.  Affinity capillary electrophoresis with laser induced fluorescence detection for thrombin analysis using nuclease-resistant RNA aptamers, Lihua Hao, Yunlong Bai, Hailin Wang, Qiang Zhao* , Journal of Chromatography A, 2016, 1476, 124-129.

18.  Determination of the platelet-derived growth factor BB by a competitive thrombin-linked aptamer-based fluorometric assay, Limin Guo, Qiang Zhao*, Microchimica Acta, 2016, 183, 3229-3235.

19.  An aptamer assay using rolling circle amplification coupled with thrombin catalysis for protein detection, Limin Guo, Lihua Hao, Qiang Zhao*, Analytical Bioanalytical Chemistry, 2016, 408, 4715-4722.

20.  Thrombin-linked aptamer assay for detection of platelet derived growth factor BB on magnetic beads in a sandwich format, Limin Guo, Qiang Zhao*, Talanta, 2016, 158, 159-164.

21.  Microplate based assay for thrombin detection using an RNA aptamer as affinity ligand and cleavage of a chromogenic or a fluorogenic peptide substrate, Lihua Hao, Qiang Zhao*, Microchimica Acta, 2016, 183, 1891-1898.

22.  Fluorescence Anisotropy Assay for D-Vasopressin with Tetramethylrhodamine-Labeled Aptamer, Yingxiong Liu, Qiang Zhao*, Analytical Methods, 2016, 8, 2383-2390.

23.  Using Fluoro Modified RNA Aptamers as Affinity Ligands on Magnetic Beads for Sensitive Thrombin Detection through Affinity Capture and Thrombin Catalysis, Lihua Hao, Qiang Zhao*, Analytical Methods, 2016, 8, 510-516.

24.  Nanoparticles-Free Fluorescence Anisotropy Amplification Assay for Detection of RNA Nucleotide-Cleaving DNAzyme Activity, Dapeng Zhang, Rong Fu, Qiang Zhao, Haiqin Rong, Hailin Wang*, Analytical Chemistry, 2015, 87, 4903-4909.

25.  A Fluorescein Labeled Aptamer Switch for Thrombin with Fluorescence Decrease Response, Lihua Hao, Qiang Zhao*, Analytical Methods, 2015, 7, 3888-3892.

26.  Aptamer and Rolling Circle Amplification-Involved Sandwich Assay for Platelet-Derived Growth Factor-BB with Absorbance Analysis, Li Lv, Limin Guo, Qiang Zhao*, Analytical Methods, 2015, 7, 1855-1859.

27.  Aptamer Fluorescence Anisotropy Sensors for Adenosine Triphosphate by Comprehensive Screening Tetramethylrhodamine Labeled Nucleotides, Qiang Zhao*, Qin Lv, Hailin Wang, Biosensors Bioelectronics, 2015, 70, 188-193.

 

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